MC-3 사람 유점액편평세포암종 세포주에서 Caffeic Acid Phenethyl Ester 매개 세포독성에 관한 연구
Caffeic Acid Phenethyl Ester-Mediated Cytotoxicity in MC-3 Human Mucoepidermoid Carcinoma Cell Line
유현주, 조남표, 조성대,
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유현주 ( Yu Hyun-Ju ) - 전북대학교 치의학전문대학원 구강병리학교실
조남표 ( Cho Nam-Pyo ) - 전북대학교 치의학전문대학원 구강병리학교실
조성대 ( Cho Sung-Dae ) - 전북대학교 치의학전문대학원 구강병리학교실
KMID : 0829220140380060357
Abstract
Caffeic acid phenethyl ester (CAPE), a component of propolis, was reported to possess anti-inflammatory, anti-bacterial, anti-viral,and anti-tumor activities. Our aim was to investigate the effect of CAPE on apoptosis in cultured human mucoepidermoid carcinoma(MEC) cell line, MC-3. Apoptotic effects of CAPE were measured by cell viability assays, Western blotting, 4’-6-diamidino-2-phenylindole(DAPI) staining and Live/Dead assay. The result of cell viability assay showed that CAPE displayed a strong growth-inhibitory effectin a concentration-dependent manner against MC-3 cells. Consumption of CAPE resulted in pronounced increase in the cleavage ofcaspase-3 and PARP, induced nuclear condensation and fragmentation and clearly increased the number of dead cells in MC-3 cells.
CAPE also caused the increase in truncated Bid (t-Bid) and the cleavage of caspase-8 and this phenomenon was regulated by deathreceptor 5 (DR5). In addition, Phosphorylation of AKT and ERK were downregulated by CAPE. Taken together, these results suggestthat CAPE is a potent apoptosis-inducing agent in MC-3 cells.
키워드
Caffeic acid phenethyl ester (CAPE); Human mucoepidermoid carcinoma (MEC); Apoptosis; Death Receptor 5
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